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Whey Protein Isolate Bioavailability Study

Bioavailablility Study

Whey protein isolate is a high quality protein that includes the naturally occurring branched-chain amino acids (BCAAs) valine, leucine, and isoleucine, as well as arginine, citrulline and ornithine (amino acids involved in nitric oxide (NO) production) as a part of the whey protein matrix.

Three healthy male adult volunteers 29 to 33 years old participated in a clinical trial to study the bioavailability of Whey Protein Isolate, a dietary supplement from NOW® Sports (# 2172). Plasma amino acid concentrations were measured at baseline, and 1, 2, and 4 hours following administration of 28 grams of Whey Protein Isolate providing 25 grams of protein.


All amino acids peaked one hour following supplementation, reaching an average of 106% and 30% above the baseline levels for BCAAs and NO-producing amino acids, respectively (Figures 1 and 2). All average amino acid concentrations returned to near-baseline levels four hours post-supplementation. The study confirms the bioavailability of BCAAs and amino acids involved in nitric oxide production from NOW® Sports Whey Protein Isolate.

In a pharmacokinetic study with three healthy male adult volunteers (29 to 33 years old), oral administration of a single serving of Whey Protein Isolate (28 grams, NOW® Sports Product # 2172) resulted in a significant increase in BCAAs (Figure 1) and amino acids involved in nitric oxide production (Figure 2), all with Tmax of one hour.

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